Sains Malaysiana 53(6)(2024): 1363-1375

http://doi.org/10.17576/jsm-2024-5306-11

Aktiviti Antibakteria Lactiplantibacillus sp. SUK1-Sumber Susu Manusiadan Produk Bakteriosin Separa Tulen terhadap Porphyromonas gingivalis

(Antibacterial Activity of  Lactiplantibacillus sp. SUK1-derived Human Milk and its Semi-pure Bacteriocin Product against Porphyromonas gingivalis)

ZALEHA SHAFIEI¹, NORAZIAH MOHAMAD ZIN^2,*, NUR DINI MUHAMAD ADLI², FATIN NUR MAZIDA SALWA MAZLAN², MOHD NIZAM LANI³ & ZAMIRAH ZAINAL ABIDIN¹

¹Jabatan Diagnostik Kraniofasial dan Biosains, Fakulti Pergigian, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

²Pusat Kajian Diagnostik, Teraputik & Penyiasatan, Fakulti Sains Kesihatan, Universiti Kebangsaan Malaysia 50300 Kuala Lumpur, Malaysia

³Fakulti Perikanan dan Sains Makanan,Universiti Malaysia Terengganu, 21030 Kuala Nerus Terengganu Malaysia

Diserahkan: 31 Oktober 2023/Diterima: 16 Mei 2024

Abstrak

Penemuan probiotik daripada susu badan manusia dapat memberi manfaat dalam mengurangkan kebolehjangkitan P. gingivalis. Kajian ini dijalankan untuk mengenal pasti potensi Lactiplantibacillus spp. yang dipencil daripada susu badan manusia untuk mengurangkan kebolehjangkitan P. gingivalis seterusnya mencirikan Lactiplantibacillus sp. yang paling berpotensi mempunyai aktiviti antibakteria. Sepuluh koloni spesies Lactiplantibacillus dengan ciri Gram-positif berbentuk bacillus, katalase negatif dan oksidase negatif dikenal pasti, namun hanya tiga koloni telah dipilih yang dikenali sebagai Lactiplantibacillus sp. SUK1, SUK2, SUK3 dan diuji aktiviti antibakteria terhadap P. gingivalis. Larutan kultur sel Lactiplantibacillus sp. SUK1 menunjukkan aktiviti perencatan yang kuat pada ujian Spot Lawn (29.33 ± 1.16 mm) dan difusi telaga agar (51.33 ± 4.73 mm), tetapi tiada aktiviti dalam ujian difusi telaga agar yang menggunakan supernatan tanpa sel yang dineutralkan dan yang tidak dineutralkan. Walau bagaimanapun, supernatan tanpa sel yang dipekatkan menunjukkan zon perencatan (13.0 ± 0.07 mm). Bakteriosin separa tulennya yang diektrak daripada pemendakan ammnonia sulfat (80%) telah merencat P. gingivalis secara berkesan, dengan pertumbuhan terendah sebanyak 40.3% pada kepekatan bakteriosin 0.8%. Analisis penjujukan 16S rRNA mencirikan Lactiplantibacillus sp. SUK1 sebagai Lactiplantibacillus pentosus strain 124-2 dengan 99.87% persamaan. Lactiplantibacillus sp. SUK1 yang dipencil daripada susu badan manusia mempunyai ciri probiotik dengan merencat pertumbuhan P. gingivalis dan bakteriosinnya dapat digunakan sebagai bahan alternatif dalam produk penjagaan kebersihan mulut bagi mencegah dan merawat penyakit periodontium.

Kata kunci: Antibakteria, bakteriosin separa tulen; Lactiplantibacillus sp. SUK1; penjujukan 16S rRNA; Porphyromonas gingivalis

Abstract

The discovery of probiotics from human milk could be beneficial to prevent chronic periodontitis by controlling P. gingivalis infection. This study was conducted to identify the potential of Lactiplantibacillus spp. isolated from human milk with antibacterial activities in reducing the infectivity of P. gingivalis and followed by the characterisation of the most potent Lactiplantibacillus sp. Ten colonies of Lactiplantibacillus species with characteristics of Gram-positive bacteria in the form of bacillus, catalase-negative and oxidase-negative were identified, however, only three colonies were selected and designated as Lactiplantibacillus sp. SUK1, SUK2, SUK3, and tested the antibacterial activity against P. gingivalis. Lactiplantibacillus sp. SUK1 cells culture suspension showed strong inhibitory activity in the Spot Lawn test (29.33 ± 1.16 mm) and agar well diffusion assay (51.33 ± 4.73 mm), but no activity in agar well diffusion assay using neutralised and non-neutralised cell-free supernatants. However, the concentrated cell-free supernatant exhibited the zone of inhibition (13.0 ± 0.07 mm). The partially purified bacteriocin extracted from ammonium sulphate precipitation (80%) effectively inhibited P. gingivalis, with the lowest growth of 40.3% at 0.8% concentration of the bacteriocin. The 16S rRNA sequencing analysis characterised the Lactiplantibacillus sp. SUK1 as Lactiplantibacillus pentosus strain 124-2 with a 99.87% similarity. In conclusion, Lactiplantibacillus sp. SUK1 isolated from human milk has probiotic properties inhibiting P. gingivalis growth and its bacteriocin could be used as an alternative material for oral hygiene care products to prevent and treat periodontal disease.

Keywords: Antibacteria; Lactiplantibacillus sp. SUK1; partial purified bacteriocin; Porphyromonas gingivalis; 16S rRNA sequencing

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*Pengarang untuk surat-menyurat; email: noraziah.zin@ukm.edu.my